Hot Tubules Velocimeter

GaN laser self-mixing velocimeter for measuring slow flows.

The self-mixing (SM) laser sensing technique allows for a simple, self-aligned, and robust system for measuring velocity. Low-cost blue emitting GaN laser diodes have recently become available owing to the high volume requirements for Blu-ray Disc devices such as high-definition video players and gaming consoles. These GaN lasers have a significantly shorter wavelength (around 405 nm) compared ...

Scissors for autolysosome tubules.

Autophagic lysosome reformation (ALR) is a cellular process in which lysosomes are reformed through scission of protolysosomes from tubular structures extruded from autolysosomes. Despite recent progress, the molecular mechanism of ALR is far from clear. A paper in this issue of The EMBO Journal has identified lysosome-localized PI(3)P, which is generated by the VPS34–UVRAG complex in an mTOR-d...

Superficial Proximal Straight Tubules

This paper describes experiments designed to evaluate Na+ and C1transport in isolated proximal straight tubules from rabbit kidneys. When the perfusing solution was Krebs-Ringer buffer with 25 mM HCO8 (KRB) and the bath contained KRB plus 6 % albumin, net volume reabsorption (J,, nl min-' mm-') was -0.46 4 0.03 (SEM); V., the spontaneous transepithelial potential difference, was -1.13 + 0.05 mV...

Melting of phospholipid tubules.

Kidney Cortex Tubules

Triacylglycerol metabolism has been studied in kidney cortex tubules from starved rats, prepared by collagenase treatment. Triacylglycerol was determined by a newly developed fully enzymic method. Incubation of tubules in the absence of fatty acids led to a decrease of endogenous triacylglycerol by about 50% in 1 h. Addition of albuminbound oleate or palmitate resulted in a steady increase of t...